Zhu, W*; Nardone, AJ; & Pearce, LA. Advances in Human Bifunctional Glutamine-Hydrolyzing Synthetases and Their Therapeutic Potential." Frontiers in Chemical Biology, 2024, 3: 1410435.
Zhu, W*, Knoll, P* , & Steinbock, O.* Exploring the Synthesis of Self-Organization and Active Motion. The Journal of Physical Chemistry Letters, 2024, 15, 5476-5487.
Cheek, LE & Zhu, W* Structural Features and Substrate Engagement in Peptide-Modifying Radical SAM Enzymes. Archives of Biochemistry and Biophysics, 2024, 110012.
Prior FSU:
Zhu, W*; Iavarone, AT; Klinman, JP* Hydrogen–deuterium exchange mass spectrometry identifies local and long-distance interactions within the multicomponent radical SAM enzyme, PqqE. ACS Central Science, 2024, 10 (2), 251-263.
Zhu, W; Klinman, JP. Biogenesis of the peptide-derived redox cofactor pyrroloquinoline quinone. Current Opinion in Chemical Biology, 2020, 59, 93-103.
Radadiya, A; Zhu, W; Coricello, A; Alcaro, S; Richards, NGJ. Improving the treatment of acute lymphoblastic leukemia. Biochemistry 2020, 59 (35), 3193-3200.
Zhu, W; Walker, LM; Tao, L; Iavarone, AT; Wei, X; Britt, RD; Elliott, SJ; Klinman, JP. Structural properties and catalytic implications of the SPASM domain iron–sulfur clusters in Methylorubrum extorquens PqqE. Journal of the American Chemical Society 2020, 142 (29), 12620-12634.
Zhu, W; Liu, X; Hughes, M; de Crécy-Lagard, V; Richards, NGJ. Whole-genome sequence of Streptomyces kaniharaensis Shomura and Niida SF-557. Microbiology Resource Announcements 2020, 9 (14), e01434-19.
Gao, S; Radadiya, A; Li, W; Liu, H; Zhu, W; de Crécy-Lagard, V; Richards, NGJ; Nasmith, JH. Uncovering the chemistry of C–C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex. Chemical Communications 2020, 56 (55), 7617-7620.
Tao, L; Zhu, W; Klinman, JP; Britt, RD. Electron paramagnetic resonance spectroscopic identification of the Fe–S clusters in the SPASM domain-containing radical SAM enzyme PqqE. Biochemistry 2019, 58 (51), 5173-5187.
Zhu, W; Radadiya, A; Bisson, C; Wenzel, S; Nordin, BE; Martínez-Márquez, F; Imasaki, T; Sedelnikova, SE; Coricello, A; Baumann, P; Berry, AH; Nomanbhoy, TK; Kozarich, JW; Jin, Y; Rice, DW; Takagi, Y; Richards NGJ, High-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivity. Communications Biology 2019, 2 (1), 1-14.
Gao, S; Liu, H; de Crécy-Lagard, V; Zhu, W; Richards, NGJ; Naismith, JH. PMP–diketopiperazine adducts form at the active site of a PLP-dependent enzyme involved in formycin biosynthesis. Chemical Communications 2019, 55 (96), 14502-14505.
Zhu, W; Reinhardt, LA; Richards, NGJ. Second-shell hydrogen bond impacts transition-state structure in Bacillus subtilis oxalate decarboxylase. Biochemistry 2019, 57 (24), 3425-3432.
Zhu, W; Martins, AM; Klinman, JP. Methods for expression, purification, and characterization of PqqE, a radical SAM enzyme in the PQQ biosynthetic pathway. Methods in Enzymology 2018, 606, 389-420
Sheng, X; Zhu, W; Huddleston, J; Xiang, DF; Raushel, FM; Richards, NGJ; Himo, F. A combined experimental-theoretical study of the LigW-catalyzed decarboxylation of 5-carboxyvanillate in the metabolic pathway for lignin degradation. ACS Catalysis 2018, 7 (8), 4968-4974.
Zhu, W; Richards, NGJ. Biological functions controlled by manganese redox changes in mononuclear Mn-dependent enzymes. Essays in Biochemistry 2017, 61 (2), 259-270.
Zhu, W; Easthon, LM; Reinhardt, LA; Tu, C; Cohen, SE; Silverman, DN; Allen, KN; Richards, NGJ. Substrate binding mode and molecular basis of a specificity switch in oxalate decarboxylase. Biochemistry 2017, 55 (14), 2163-2173.
Zhu, W; Wilcoxen, J; Britt, RD; Richards, NGJ. Formation of hexacoordinate Mn(III) in Bacillus subtilis oxalate decarboxylase requires catalytic turnover. Biochemistry 2016, 55 (3), 429-434.
Tang, RC; Guan, Z; He, YH; Zhu, W. Enzyme-catalyzed Henry (nitroaldol) reaction. Journal of Molecular Catalysis B: Enzymatic 2013, 63 (1-2), 62-67.
Conference Presentations
Jul. 2023 - Mapping Protein-Protein Interactions in a Multi-Component Radical SAM Enzyme, Metallocofactors Gordon Research Conference, South Hadley, MA (Poster Talk)
Jun. 2023 -Catalytic mechanism of radical SAM enzyme PqqE in pyrroloquinoline quinone biosynthesis, 99th Florida Annual Meeting and Exposition, FL (Lecture)
Dec. 2021 - Catalytic mechanism of the radical SAM enzyme in PQQ biosynthesis, Reactivity and Mechanism in Chemical and Synthetic Biology, Pacifichem 2021, Honolulu, HA (Lecture)
Sept. 2020 - Characterization of iron-sulfur clusters in radical SAM enzyme PqqE in PQQ biosynthesis, ACS #ChemistsLive ACS Cross-Division Virtual Live Content Event (Lecture)
Jun. 2018 - Identification and characterization of the Fe-S clusters in PqqE in pyrroloquinoline quinone biosynthetic pathway, Metallocofactors Gordon Research Conference, South Hadley, MA (Poster)
May 2017 - Application of membrane inlet mass spectrometry in the mechanistic study of LigW-catalyzed decarboxylation of 5-carboxyvanillate, 17th Cardiff Chemistry Conference, Cardiff, UK (Lecture)
Sept. 2016 - Molecular basis of a specificity switch in oxalate decarboxylase/oxalate oxidase chimeric variant, EMBO Conference Chemical Biology 2016, Heidelberg, Germany (Poster)
Jun 2016 - How does a metalloprotein catalyze a chemically difficult reaction: the mechanistic study of Bacillus subtilis oxalate decarboxylase, 4th Royal Chemical Society Early Career Symposium, Glasgow, UK (Lecture)
Sept. 2015 - Substrate-binding facilitates Mn(III) formation in Bacillus subtilis oxalate decarboxylase, 35th Midwest Enzyme Chemistry Conference, Chicago, IL (Lecture)
Sept 2014 - Exploration of the mechanism of oxalate decarboxylase by heavy atom kinetic isotope effects, 34th Midwest Enzyme Chemistry Conference, Evanston, IL (Poster)
May 2014 - Primary 13C kinetic isotope effects as a probe of the mechanism of oxalate decarboxylase, 36th Steenbock Symposium Enzyme Structure and Function, Madison, WI (Poster)
Sept. 2013 - Investigating the functional importance of N-terminal Mn(II) center loop residues in oxalate decarboxylase, 33th Midwest Enzyme Chemistry Conference, Chicago, IL (Poster)
Sept. 2013 - Investigating the functional importance of active site loop residues in switching the reactivity of the Mn(II) center in oxalate decarboxylase, 246th American Chemistry Society National Meeting and Exposition, Indianapolis, IN (Poster)
Jul. 2013 - Characterizing the catalytic properties of the oxalate decarboxylase/oxalate oxidase chimeric mutant, Gordon Research Conference: Enzymes, Coenzymes & Metabolic Pathways, Waterville Valley, NH (Poster)